产品介绍 目录号
Serial | GNHu17 | 标识符
Identifier | CSTR:19375.09.3101HUMGNHu17 | 描述
Description | 293细胞株插入SV40 T-抗原的温度敏感基因后产生的高转染效率的衍生株称为293T。
在本库通过支原体检测。
在本库通过STR检测。 | 动物种别
Organism | 人 | 性别
Gender | *** | 组织来源
Tissue and Cell Type | 肾 | 形态
Morphology | 上皮细胞 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Sena-Esteves M , et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001Pear WS , et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960 |
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