试剂仪器设备原料
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细胞培养基 >> 细胞株
ACHN人肾腺癌细胞(2019年新引进)
ACHN人肾腺癌细胞(2019年新引进)图片
产品介绍
目录号
Serial
SCSP-5063
标识符
Identifier
CSTR:19375.09.3101HUMSCSP5063
描述
Description
细胞株始建于1979年11月,源于一名22岁的白种人男性恶性胸腔积液,他患有广泛转移的肾腺癌。该细胞的生长受到人干扰素抑制,应用于人干扰素或干扰素诱导物的抗增殖研究。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测无误。STR鉴定结果为:Amelogenin: X;CSF1PO: 11;D13S317: 12;D16S539: 12,13;D5S818: 12;D7S820: 9,11;THO1:8;TPOX: 8,11;vWA: 16,17。
动物种别
Organism
人;白人
性别
Gender
男性,22岁
组织来源
Tissue and Cell Type
肾脏;来源于转移部位:胸腔积液
形态
Morphology
上皮样,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
人肾腺癌细胞ACHN完全培养液配方(100 ml):
MEM (Gibco, 110900081)87 ml
Glutamax (Gibco, 35050-061) 1 ml
Sodium Pyruvate (Gibco, 11360-070) 1 ml
Non-essential Amino Acids, 100 (Invitrogen, 11140050)  1 ml
FBS (Gibco)10 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
生物安全等级:BSL-1
参考传代比例:1:2-1:3
参考传代周期:3-5天
参考换液频率:每周3次
冻存液配方:完全培养液95%,DMSO 5%
【注意事项】
该细胞生长受到人类干扰素抑制
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCETumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons. Kochevar J. Blockage of autonomous growth of ACHN cells by anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res. 20: 2968-2972, 1990. PubMed: 2334900
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