产品介绍 目录号
Serial | SCSP-509 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP509 | 描述
Description | 虽然原始肿瘤具有神经元和神经胶质分化的特征，但该细胞没有保留这些特征。用双丁酰环磷腺苷(cAMP)处理后通过S100(S-100)蛋白和胶质原纤维酸性蛋白(GFAP)染色，发现该细胞不会诱导上述特征的表达。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X; CSF1PO: 11; D13S317: 13,14; D16S539: 10; D5S818: 11,13; D7S820: 8,10; THO1: 9; TPOX: 8,10; vWA: 14,20 | 动物种别
Organism | 人；白人 | 性别
Gender | 男性，4岁 | 组织来源
Tissue and Cell Type | 大脑/小脑 | 形态
Morphology | 多边形，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人髓母细胞瘤细胞 Daoy 完全培养基配方(100 ml)：
MEM (Invitrogen，11090081) 87 ml
FBS (Gibco)10 ml
Glutamax(invitrogen 35050061) 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373Jacobsen PF, et al. Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice. J. Neuropathol. Exp. Neurol. 44: 472-485, 1985. PubMed: 2993532Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 |
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