试剂仪器设备原料
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细胞培养基 >> 细胞株
HCC827人非小细胞肺癌细胞
HCC827人非小细胞肺癌细胞图片
产品介绍
目录号
Serial
TCHu153
标识符
Identifier
CSTR:19375.09.3101HUMTCHu153
描述
Description
这株细胞建于1994年三月。这株肺腺癌在EGFR酪氨酸激酶区域有一个获得性突变(E746-A750缺失)。 患者在25岁到26岁时每个月抽1包烟。 在诊断前12年不再抽烟。
在本库通过支原体检测。
在本库通过STR检测。
动物种别
Organism
性别
Gender
组织来源
Tissue and Cell Type
肺腺癌
形态
Morphology
上皮细胞样
培养基和添加剂
Complete Growth Medium and Culture Conditions
RPMI 1640培养基(GIBCO by life technologies ,货号C11875500BT),90%;优质胎牛血清,10%。
气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCEGirard L , et al. Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering. Cancer Res. 60: 4894-4906, 2000. PubMed: 10987304Burbee DG , et al. Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression. J. Natl. Cancer Inst. 93: 691-699, 2001. PubMed: 11333291Toyooka S , et al. Differential expression of FEZ1/LZTS1 gene in lung cancers and their cell cultures. Clin. Cancer Res. 8: PubMed: 12114433Virmani A , et al. Aberrant methylation of the cyclin D2 promoter in primary small cell, nonsmall cell lung and breast cancers. Int. J. Cancer 107: 341-345, 2003. PubMed: 14506731Liu CX , et al. LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines. Cancer Res. 60: 1961-1967, 2000. PubMed: 10766186Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L.,  Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.
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