产品介绍 目录号 Serial | TCHu106 | 标识符 Identifier | CSTR:19375.09.3101HUMTCHu106 | 描述 Description | 该细胞系来自8岁男性黑人的组织。 其染色体模式数目为60,在裸鼠中能致瘤。 已知该细胞系含有与人类疾病有关的因子,需在2级生物安全防护下操作。 在本库通过支原体检测。 在本库通过STR检测。 | 动物种别 Organism | 人 | 性别 Gender | 男 | 组织来源 Tissue and Cell Type | 肝细胞癌,肝 | 形态 Morphology | 上皮细胞 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Knowles BB , et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960Knowles BB , Aden DP . Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983Aden DP , et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137Darlington GJ , et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851Cancer Research 63:3775-3782,2003Chinese Journal of Cancer 21(3):267-271, 2002 |
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