产品介绍 目录号
Serial | SCSP-532 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP532 | 描述
Description | 人前列腺癌细胞PC-3来源于一名62岁IV级前列腺癌白人男性患者的骨髓转移灶。此细胞酸性磷酸酶(acid phosphatase)和5-α-睾丸激素还原酶(testosterone-5-alpha reductase) 活性表达低。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X；CSF1PO: 11；D13S317: 11；D16S539: 11；D5S818: 13；D7S820: 8,11；THO1: 6,7；TPOX: 8,9；vWA: 17 | 动物种别
Organism | 人；白人 | 性别
Gender | 男性，62岁 | 组织来源
Tissue and Cell Type | 前列腺；分离自转移灶：骨 | 形态
Morphology | 上皮样，贴壁生长；该细胞在软琼脂中成蔟生长，并可适应悬浮培养) | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人前列腺癌细胞PC-3细胞完全培养液配方(100 ml)：
Ham's F-12K (Kaighn's) Medium (Invitrogen, 21127-022) 89 ml
FBS (Gibco) 10 ml
Glutamax (Invitrogen, 35050061) 1 ml
参考传代周期：3-4天
参考传代比例：1:3—1:6
参考换液频率：每周2-3次
冻存液配方：完全培养液95%，DMSO 5%
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Kaighn ME, et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. PubMed: 447482 Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet. Cell Genet. 62: 183-184, 1993. PubMed: 8428522 Ohnuki Y, et al. Chromosomal analysis of human prostatic adenocarcinoma cell lines. Cancer Res. 40: 524-534, 1980. PubMed: 7471073 Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194 Umekita Y, et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218 Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628 Nupponen NN, et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 9460501 Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Su ZZ, et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 8692978 |
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