产品介绍 目录号
Serial | SCSP-5029 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP5029 | 描述
Description | 人神经母细胞瘤细胞SK-N-SH由J.L.Bieder建系。与SK-N-MC所不同的是，SK-N-SH细胞倍增时间较长且多巴胺-β-羟基酶水平较高。SK-N-SH在细胞介导的细胞毒性试验中用作靶细胞系。我库冻存时为P42代。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：Amelogenin: X；CSF1PO: 11；D13S317: 11；D16S539: 8,13；D5S818: 12；D7S820: 7,10；THO1: 7,10；TPOX: 8,11；vWA: 14,18 | 动物种别
Organism | 人 | 性别
Gender | 女性，4岁 | 组织来源
Tissue and Cell Type | 脑；来自转移灶：骨髓 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人神经母细胞瘤细胞SK-N-SH完全培养液配方(100 ml)：
MEM(invitrogen 11090081)87 ml
FBS(Gibco)10 ml
Gluta-max(invitrogen 35050061) 1 ml
NEAA (Invitrogen, 11140050) 1 ml
Sodium pyruvate(invitrogen 11360070) 1 ml
参考传代周期：3～5天一次
参考传代比例：1:3-1:5
参考换液频率：每周2次
冻存液配方：完全培养液95%，DMSO 5%
气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Gilbert LC, Wachsman JT. Characterization and partial purification of the plasminogen activator from human neuroblastoma cell line, SK-N-SH. A comparison with human urokinase. Biochim. Biophys. Acta 704: 450-460, 1982. PubMed: 7052133Spengler BA, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells established in vitro. In Vitro 8: 410, 1973.Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013Seeger RC, et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461Yan SD, et al. Amyloid-beta peptide-Receptor for Advanced Glycation Endproduct interaction elicits neuronal expression of macrophage-colony stimulating factor: A proinflammatory pathway in Alzheimer disease. Proc. Natl. Acad. Sci. USA 94: 5296-5301, 1997. PubMed: 9144231Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024Chang YE, et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed: 8648731He B, et al. The carboxyl terminus of the murine MyD116 gene substitutes for the corresponding domain of the gamma134.5 gene of herpes simplex virus to preclude the premature shutoff of total protein synthesis in infected human cells. J. Virol. 70: 84-90, 1996. PubMed: 8523596Yoshikawa T, et al. Downstream regulatory elements increase acute and latent herpes simplex virus type 2 latency-associated transcript expression but do not influence recurrence phenotype or establishment of latency. J. Virol. 70: 1535-1541, 1996. PubMed:8627672 |
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