产品介绍 目录号
Serial | SCSP-217 | 标识符
Identifier | CSTR:19375.09.3101MOUSCSP217 | 描述
Description | 该细胞系已广泛用于创建基因敲除小鼠。HPRT负突变使该品系可用于染色体工程。已经表明该小鼠ES细胞系具有生殖能力。每个批次均通过本库支原体检测，结果为阴性。我库冻存时为P24代。
培养时需使用CF-1 MEF(SCSP-105R)作为饲养层细胞。 | 动物种别
Organism | 小鼠，129S5/SvEvBrd(原129/SvEvBrd)品系 | 性别
Gender | 雄性 | 组织来源
Tissue and Cell Type | 小鼠胚胎内细胞团 | 形态
Morphology | 球形克隆 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | mES完全培养液配方(600 ml)：
DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985) 1.5 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Bader A, Al-Dubai H, Weitzer G. Leukemia Inhibitory Factor Modulates Cardiogenesis in Embryoid Bodies in Opposite Fashions. Circ. Res. 86: 787-794, 2000. PubMed: 10764413 Wolcik SM, Longley MA, Roop DR. Discovery of a novel murine keratin 6 (K6) isoform explains the absence of hair and nail defects in mice deficient for K6a and K6b. J. Cell Biol. 154: 619-630, 2001. PubMed: 11489919 Liu Y, et al. Sox17 is essential for the specification of cardiac mesoderm in embryonic stem cells. Proc Natl Acad Sci USA. 104(10): 3859-3864, 2007. PubMed: 17360443 Adams DJ, et al. A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction. Genomics 86(6): 753-758. 2005. PubMed: 16257172Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999.PubMed: 10430671 Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391 Bradley A, Zheng B, Liu P. Thirteen years of manipulating the mouse genome: a personal history. Int. J. Dev. Biol. 42 (7): 943-950, 1998. PubMed: 9853825 |
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