细胞培养基 >> 细胞株
Neuro-2a [N2a, Neuro-2a]小鼠脑神经瘤细胞
产品介绍 目录号
Serial | TCM29 | 标识符
Identifier | CSTR:19375.09.3101MOUTCM29 | 描述
Description | 克隆Neuro-2A是由R.J.Klebe和F.H.Ruddle经A株白鼠的自生肿瘤建立。 该细胞产生大量微管蛋白，此蛋白在神经细胞中起应答轴浆流动的收缩系统作用。
在本库通过支原体检测。 | 动物种别
Organism | 小鼠 | 性别
Gender | 不明 | 组织来源
Tissue and Cell Type | 脑神经母细胞瘤，神经母细胞 | 形态
Morphology | 神经和阿米巴样干细胞，主要贴壁生长、较少部分半贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Olmsted JB , et al. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc. Natl. Acad. Sci. USA 65: 129-136, 1970. PubMed: 5263744Klebe RJ , Ruddle FH . Neuroblastoma: Cell culture analysis of a differentiating stem cell system. J. Cell Biol. 43: 69A, 1969.Naslavsky N , et al. Characterization of detergent-insoluble complexes containing the cellular prion protein and its scrapie isoform. J. Biol. Chem. 272: 6324-6331, 1997. PubMed: 9045652Kaneko K , et al. Evidence for protein X binding to a discontinuous epitope on the cellular prion protein during scrapie prion propagation. Proc. Natl. Acad. Sci. USA 94: 10069-10074, 1997. PubMed: 9294164Maestrini E , et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614 |
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